Cross-linking mass spectrometry for mapping protein complex topologies in situ

K Lee, FJ O'Reilly - Essays in Biochemistry, 2023 - portlandpress.com
… , the cross-linker may require additional functionality in its spacer … are highly reactive with
lysines and the N-termini of proteins, with … model that placed NusA into the unexplained density. …

[HTML][HTML] A composite filter for low FDR of protein-protein interactions detected by in vivo cross-linking

L de Jong, W Roseboom, G Kramer - Journal of proteomics, 2021 - Elsevier
… of the azido group in the spacer of the cross-linker to an amine … protein L17 (RL17_BACSU),
which is located in closecross-linker for facile mapping of lysines in close proximity

Cross-link scrambling in peptide pairs

L de Jong, W Roseboom, G Kramer - bioRxiv, 2022 - biorxiv.org
… the azido group in the spacer of the cross-linker to an amine … lacks a lysine residue and
does not contain the protein N-… Nevertheless according to its SCX retention time the peptide …

[HTML][HTML] Towards low false discovery rate estimation for protein-protein interactions detected by chemical cross-linking

L de Jong, W Roseboom, G Kramer - … et Biophysica Acta (BBA)-Proteins …, 2021 - Elsevier
… N-hydroxysuccinimidyl ester groups connected by a spacer. … inter-protein peptide pairs using
the cleavable cross-linker DSBU … Of the 6 novel interactions, 5 can be placed in a regulatory …

A solid-phase approach for the synthesis of muramyl dipeptide conjugates for detection of NOD2

I Kekessie, T Goncharov, LG Kőműves, D Vucic… - Bioorganic …, 2021 - Elsevier
… Our solid phase approach synthetically provides a facile … mechanism are specialized
groups of proteins that recognize … The azido group was converted to an amino group by …

Photoluminescent nanoparticles for chemical and biological analysis and imaging

WR Algar, M Massey, K Rees, R Higgins… - Chemical …, 2021 - ACS Publications
… through molecular design and protein engineering is finite. … , leading to relatively facile
intersystem crossing during MLCT … for trap states located energetically within the band gap and …